To determine entrapment efficiency of sparfloxacin lactate liposome with cation exchange resin-HPLC

摘要

OBJECTIVE: To determine the entrapment efficiency of sparfloxacin lactate liposome. METHOD: The cation exchange resin was adopted to separate free sparfloxcain lactate from liposome dispersions. The content of sparfloxacin was determined by HPLC with Hypersil ODS2 column (250mm×4.6mm, 5μm). The mobile phase was 0.2mol·L-1 ammonium acetate solution acetonitrile (70:30 v/v, adjusted to pH 3.5 with acetic acid). The flow rate and UV detection were set respectively at 1.0mL·min-1 and 298nm. RESULTS: The free sparfloxacin was well separated from liposome dispersions and was adsorbed up to 9643% by cation exchange resin, but liposome was less than 2.63%. Sparfloxacin had a good linear relation in the range of 4.2-50.4μg·mL-1 (R2=1), the precision (RSD) was less than 1.30%, the sample recoveries were 99.96%-102.11%. CONCLUSION: This method is technically simple, rapid and efficient to separate and determine entrapment efficiency of sparfloxacin liposome.