Generation of Pesticides-degrading Genetically Engineered Bacteria Expressing the Protein of GFP and Carboxylesterase B1

摘要

The genetically engineered bacteria, expressing the fusion protein of enhanced green fluorescent protein (EGFP) and carboxylesterase B1, have been successfully constructed, by cloning the genes of EGFP and carboxylesterase B1 into the pET-28b vector and then transforming E. coli BL21 (DE3). Expression of the fusion protein can be induced in E. coli BL21 (DE3) at 28°C for 8h by even as little as 0.01mM isopropyl β-D-thiogalactopyranoside (IPTG). The genetically engineered bacteria not only can degrade pesticides in the environment but also can be found easily by fluorescence spectrophotometry. The green fluorescence emitted by EGFP in genetically engineered bacteria can be seen even by naked eye in daylight.