Electrochemical Study of A DNA Base Probe Based on Anticancer Drug Dacarbazine - Subtitle: A DNA Base Probe

摘要

An anticancer drug dacarbazine (DTIC) was immobilized on a glassy carbon electrode (GCE) by using electrochemical oxidation method. Then the DTIC-modified GCE was utilized as a stable functional monolayer to further associate with DNA bases and the different blocking effects on the redox reaction of (Fe(CN){sub}6){sup}3- were observed on the different DNA base associated DTIC/GCE, where much bigger blocking effects on the electron transfer of (Fe(CN){sub}6){sup}3- appear on the purines/DTIC/GCE. Based on these observations, the single base change of the oligonucleotides by using the relative modified electrode has been further explored, and the results illustrate that the single base change in DNA sequences could be sensitively recognized by the related probe. Besides, our results also indicate that the specific binding of DTIC to different DNA bases could dramatically change the important interfacial characteristics of the relative surface, which may be utilized as a novel way for the DNA base detection and imply a potential application as a sensitive probe for some important biological process.