A Behavior of Protein Adsorption on Poly(Ethylene Glycol)-Modified Surfaces under Flow Conditions at Relatively Low Concentrations for Microfluidics Systems

摘要

As devices, especially biomedical devices, decrease in size, their surface-to-volume ratio increases proportionally. Combined with handling of relatively smaller amount of analytes at low concentrations, the problem of analyte streams depletion due to adsorption to the device surface prior to detector arises. Thus, the basic understanding of an adsorption behavior of biomolecules on the surfaces of these systems is critical for their use in microfluidics as well as bioanalytical devices. Protein adsorption of alkaline phosphatase (AP) and horseradish peroxidase (HRP) onto poly(ethylene glycol) (PEG)-modified microcapillary surfaces has been determined under flow conditions at different flow rates and protein concentrations ranging from 10-300 ng/ml determined. The flow rate affected the protein adsorption on the surfaces at a fixed protein concentration;