Microarray analysis of the cell cycle-related geneexpression from a RAW264 murine macrophagecell line in response to lipopolysaccharide

摘要

When a murine macrophage cell line, RAW264, was stimulated with lipopolysaccharide (LPS), cell cycle arrest was observed. DNA microarray analysis clarified that out of the 99 cell cycle-related genes, LPS significantly up-regulated 4 genes; two major of which were cyclin-dependent kinase inhibitor 2D (cdkn2d) and nuclear factor of kappa light chain gene enhancer (Nfkbia), and down-regulated 1 gene, namely cyclin A2 (Ccna2). These gene expressions could directly account for the cell cycle arrest of the macrophage examined.