Breeding and Utilization of New Cytoplasmic Male Sterile Line from New Cytoplasmic Source Dongxiang Wild Rice

摘要

By hybridizing and multiple crossing the plant of Dongxiang wild rice and the cultivated variety rice, and continually backcrossing between the male sterile plants of the progeny and the cultivated variety rice, two different male sterile types have been bred after their cell nucleus finally were replaced many progenies: One is anther-degenerated abortion type: Its anther is thin, degenerated, hamate crooked and white. By iodine-iodine of potassium dying and microscopic examining, typical aborted pollen grains are very few, by test -crossing with wild aborted type of R-line, the first filial generation appears completely sterile and by test -crossing with close affinity material its restorable degree is improved, but no heterosis, such as Dong B_(51)A, Dong Pei A, Dong R 4005A and Dong 3037A etc. The other is typical abortion type, anther is fatter, milky white and water stained, by iodine-iodine of potassium dying and microscopic examining, typical aborted pollen grains are many, round aborted pollen grains are very few, its restore spectrum is very extensive , such as Dong B_(11) A. But we have separated the typical abortion type of CMS line from anther-degenerated a-bortion type of one, and we have analyzed the phenomenon. Dong B_(11)A has already been technically indentified and authenticated in our province, its major morphogical and biological characteristics have been interpreted, its combining ability and heterosis in exploition have been tested, a plenty of better and newer combinations have been selectively bred after widely test - crossing, such as Dongyou 13, Dongyou 952 and Dongyou C 218 ect.