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Molecular identification of the cytoplasmic male sterile source from Dongxiang wild rice (Oryza rufipogon Griff.)

机译:东乡野生稻(Oryza rufipogon Griff。)胞质雄性不育来源的分子鉴定

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摘要

The use of cytoplasm male sterility (CMS) is crucial for three-line hybrid seed production.Two types of CMS have been discovered from Dongxiang wild rice,namely the wild abortion type CMS (CMS-WA) and the Dongxiang wild type CMS (CMS-DW).In this study,we show the molecular identification of the two types of CMS in Dongxiang wild rice.WA352,which conferred CMS-WA,was not detected in Dongxiang wild rice,implying Dongxiang wild rice does not carry the CMSWA source.Further analysis of WA352 in DY1A,a CMS-DW line,by PCR amplification and sequencing,revealed two insertion-deletion polymorphisms occurred in CMS-DW compared to WA352 of CMS-WA.It was reported that WA352 was comprised of an unknown origin sequence and partial sequences of three open reading frames (ORFs),orf284,orf224 and orf288.The 42-bp insertion was located between the two segments of orf224 and orf288,which created a new chimeric ORF,orf216.This new ORF was also detected in CMS-HL.Based on the 9-bp deletion in orf284,a specific mitochondrial marker of DW-M1 was developed,which could be used to specifically distinguish the DW-type source.Moreover,semi-quantitative RT-PCR analysis preliminarily suggested that both orf216 and orf284 could be considered as candidates for CMS-DW.These findings present a preliminary understanding of CMS-DW at the molecular level.
机译:利用细胞质雄性不育(CMS)对于三系杂交种子的生产至关重要。从东乡野生稻中发现了两种类型的CMS,即野生流产型CMS(CMS-WA)和东乡野生型CMS(CMS)。 -DW)。在本研究中,我们显示了东乡野生稻中两种类型CMS的分子鉴定。在东乡野生稻中未检出赋予CMS-WA的WA352,这意味着东乡野生稻不携带CMSWA源。通过PCR扩增和测序进一步分析CMS-DW系DY1A中的WA352,与CMS-WA的WA352相比,揭示了CMS-DW中发生了两个插入缺失多态性。据报道,WA352的来源不明三个开放阅读框(ORF),orf284,orf224和orf288的序列和部分序列。42 bp插入位于orf224和orf288的两个片段之间,从而创建了一个新的嵌合ORF或orf216。也检测到了这个新的ORF基于CMS-HL中的9 bp缺失开发了DW-M1的线粒体标记,可用于特异性区分DW型来源。此外,半定量RT-PCR分析初步表明orf216和orf284均可被视为CMS-DW的候选对象。研究结果从分子水平对CMS-DW进行了初步了解。

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  • 来源
    《农业科学学报(英文版)》 |2017年第8期|1669-1675|共7页
  • 作者单位

    Rice National Engineering Laboratory(Nanchang)/Nanchang Sub-center,National Rice Improvement Center/Jiangxi Provincial Key Laboratory of Rice Physiology and Genetics/Rice Research Institute,Jiangxi Academy of Agricultural Sciences,Nanchang 330200,P.R.China;

    Rice National Engineering Laboratory(Nanchang)/Nanchang Sub-center,National Rice Improvement Center/Jiangxi Provincial Key Laboratory of Rice Physiology and Genetics/Rice Research Institute,Jiangxi Academy of Agricultural Sciences,Nanchang 330200,P.R.China;

    Key Laboratory of Crop Physiology,Ecology and Genetic Breeding,Ministry of Education/College of Agronomy,Jiangxi Agricultural University,Nanchang 330045,P.R.China;

    Jiangxi Super-rice Research and Development Center,Jiangxi Academy of Agricultural Sciences,Nanchang 330200,P.R.China;

    Rice National Engineering Laboratory(Nanchang)/Nanchang Sub-center,National Rice Improvement Center/Jiangxi Provincial Key Laboratory of Rice Physiology and Genetics/Rice Research Institute,Jiangxi Academy of Agricultural Sciences,Nanchang 330200,P.R.China;

    Rice National Engineering Laboratory(Nanchang)/Nanchang Sub-center,National Rice Improvement Center/Jiangxi Provincial Key Laboratory of Rice Physiology and Genetics/Rice Research Institute,Jiangxi Academy of Agricultural Sciences,Nanchang 330200,P.R.China;

    Rice National Engineering Laboratory(Nanchang)/Nanchang Sub-center,National Rice Improvement Center/Jiangxi Provincial Key Laboratory of Rice Physiology and Genetics/Rice Research Institute,Jiangxi Academy of Agricultural Sciences,Nanchang 330200,P.R.China;

    Rice National Engineering Laboratory(Nanchang)/Nanchang Sub-center,National Rice Improvement Center/Jiangxi Provincial Key Laboratory of Rice Physiology and Genetics/Rice Research Institute,Jiangxi Academy of Agricultural Sciences,Nanchang 330200,P.R.China;

  • 收录信息 中国科学引文数据库(CSCD);中国科技论文与引文数据库(CSTPCD);
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  • 入库时间 2022-08-18 00:24:00
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