Genetic Diversity Analysis of PVY-resistant Potato Accessions by RAPD Makers

摘要

DNA fingerprints of 37 PVY-resistant potato accessions were generated using PCR-based RAPD analysis. All 37 culti-vars were differentiated by banding patterns obtained from 17 primers that generated 164 polymorphisms. Similarity matrices were calculated with the simple matching coefficient. The genetic distance value among them was between 0.27 and 0.50, average value was 0.35. and the average link clustering algorithm was used to construct a dendrogram with STATISTICA. They were classified into three groups according to clustering results, and the results presented some geographic relationship. Dendrogram showed close genetic relationships between a number of cultivars of similar pedigree. This study has shown that RAPD markers is a useful tool forpotato cultivar identification, differentiation, and estimating genetic relationships.