Precision Mathematical Model with Distributed Constructional Characteristics for Vascular Endothelial Cells in Electrical Cell-substrate Impedance Sensing (ECIS) Method

摘要

We proposed a precision mathematical model for the micro-dynamics of cultured cells measured with ECIS (Electrical Cell-substrate Impedance Sensing) system. Our mathematical model is composed of culture medium impedance between cells (Z{sub}(sol)), cell impedance (Z{sub}c), and polarization impedance of the electrode (Z{sub}p). It was found that whether cell-to-cell or cell-to-substrate gaps caused the changes in the impedance could be determined based on the changes in the total resistance and reactance (capacitance). Cell-to-cell gaps mainly affect the total resistance value. Cell-to-substrate gaps mainly affect total capacitance value.