Proximal Two-Beam Optical Tweezers for Rotational Control of Living Single-Cells

摘要

Currently, we study about spectroscopy-tomography of living single-cells whose diameter is about 10μm to assist the quantitative diagnosis of early cancer. This technology is composed of two elemental technologies, high spatial resolution spectrometry and a precise single-cell rotating method. In these two technologies, we propose proximal two-beam optical tweezers as the precise rotating method. In this method, we harness the light pressure generated by light absorption as rotating torque. We decided to illuminate the proximal two points in each from different directions using two beams. In this case, the light pressure generated by light absorption is made to act as rotating torque. Using this proposed method, we can control the rotational velocity of a microsphere regardless of refractive index distribution by non-contact operation. In addition, rotational speed is controlled by optical PWM operation. This proposed optical PWM operation is that the received light intensity is changed by the illumination time. This method can be developed into the 6-DOF control of single-cell.