Blockade of keratinocyte-derived chemokine inhibits endothelial recovery and enhances plaque formation after arterial injury in apolipo-protein E-deficient mice

摘要

We evaluated the involvement of keratinocyte-derived chemokine (KC) in neointimal hyperplasia and endothelial repair following arterial injury.Wire-induced injury of common carotid arteries in apolipoprotein E-deficient (apoE_/") mice treated with either a neutralizing monoclonal anti-KC or isotype control antibody (twice weekly, 50 ug per injection, n=6 per group) for three weeks on high cholesterol diet was performed. Serial sections of the carotid artery were stained with Movats pentachrome and analyzed for arterial wall. a-SMA, Mac-2 plaque content and CD31 and VE-Cadherin positive luminal cells were determined. Carotid sections were co-stained for KC or CXCR2 and cell-specific markers (Mac-2 or CD31).