The illegal use of drugs has led to the occurrence of residues in food of animal origin which is very harmful to people's health. This work was to develop and evaluate a sensitive and fast assay to determinate sulfamethoxazole (SMOZ) in HBS and milk with surface plasmon resonance (SPR) biosensor. The derivative of SMOZ was covalently immobilized to a carboxymethyldextran modified gold film. A recently developed monoclonal antibody against sulfamethoxazole was used to develop an inhibition immunobiosensor screening assay for sulfamethoxazole in milk. The antibody was mixed with SMOZ of different concentrations prepared with HBS and milk to constructed a standard curves with a low LOD and IC50 value. The assay precision was also studied. The critical factor for the assay sensitivity was non-specific binding to the sensor chip surface from matrix, the effect of matrix on the essay was studied and was controlled by dilution. The cross-reactivity with other sulfanamides was also studied.
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