Vascularized bone marrow transplantation leads to higher levels of hematopoietic chimerism than conventional cellular bone marrow transplantation

摘要

The aim of the study was to follow the development of microchimerism after allogeneic vascularized bone marrow transplantation (VBMT) vs conventional bone marrow transplantation (BMT). Methods In one group a VBMT model consisted of a donor Brown Norway (BN) rat hind limb heterotopic transplanted on recipient Lewis rats was used. An intravenous infusion of donor bone marrow cells in suspension equivalent to that grafted in the vascularized femur limb was administrated i.v. on recipient rats in the second group. Cellular microchimerism was investigated in recipients of VBMT vs BMT. Results Donor-derived cells could be detected in VBMT recipients at 30 and 60 days but not in recipients of i.v. suspension BMC grafting. Conclusions VBMT provides a theoretical alternative to conventional cellular bone marrow transplantation by addressing crucial clinical problems such as failure of engraftment or graft versus host disease.