Tripodal thiol-derivatives as a functional interface monolayer for immobilization of biomolecules

摘要

We designed interface molecules for immobilization of biomolecules on solid substrate and applied it to genetic field effect transistors. We have been investigating a new approach to realize a potentiometric detection for DNA chips. The concept of a genetic field effect transistor has been proposed for improving precision, standardization and miniaturization of a DNA chip system. We have designed and synthesized tripodal thiol-derivatives for stable immobilization of oligonucleotide probes on a gold surface. The genetic FET platform combined with the new interface molecule is suitable for a simple, accurate and inexpensive system for SNP typing in clinical diagnostics.