Gene detection device that was used and this tripod type functional interface molecules for biomolecular immobilization

摘要

Disclosed is a tripodal functional interface molecule having bound thereto a thiol-linked atomic groups which is bound to each of three bounds of the bonds of a carbon atom in the molecule and an atomic group containing an oligonucleotide-binding group which is bound to the rest one bond of the carbon atom. The interface molecule is aligned on a substrate, and a nucleic acid is bound to the oligonucleotide-binding group on the interface molecule, thus enabling an efficient hybridization. Also disclosed is a device for detecting a gene, which has a channel section and a metal electrode provided adjacent to the channel section. A single-stranded nucleic acid probe is immobilized on the metal electrode side, hybridization is performed on the surface of the metal electrode to form double-stranded DNA, a molecularbiological process (e.g., horizontal extension) is additionally performed, and an electrical signal generated according to the change in carrier density on the surface of a semiconductor is detected. Thus, technical means for nucleic acid immobilization which can provide an inexpensive system and a device for detecting a gene utilizing the means can be provided.