The development of a novel immunosensor based on microchip zone electrophoresis in combination with fluorescence detection is described for application in the point-of-care clinical diagnosis. Wells were microfabricated on a polyacrylamide gel, which was the supporter of the microchannels on silicon substrate. In consequence to the specific reaction between anti-human albumin antibody and the labeled antigen in the wells, free and bound labeled antigen were separated by zone electrophoresis. The quantification of antigens was performed by monitoring the fluorescence signal with a microscope. Anti-human albumin antibody could selectively be detected with a linear range between 1 and 100 ng/ml in about 40 min.
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