We present a 3-D microfluidic device that enables localized drug delivery to living slime mould, a giant amoeba-like cell. The device features a flow cell in which drugs can be introduced at several positions, allowing drugs to be delivered to the slime mould at eight different locations. This is in contrast to devices reported in the literature, which allow cells to be addressed at only a few positions. Slime mould patterning is accomplished by insertion of SU-8 structures containing mould into the flow cell. This assembly allowed us to deliver drugs to the cell to evoke a measurable change in its oscillation behaviour.
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