AN INTEGRATED APPROACH TO THE CONSERVATION AND MANAGEMENT OF THE EUROPEAN EEL IN THE MEDITERRANEAN REGION: ISOLATION AND CHARACTERIZATION OF EST-LINKED MICROSATELLITE LOCI

摘要

Expressed Sequence Tags (ESTs) derived from complementary DNA (cDNA) libraries have been recently been developed for a variety offish and shellfish species. The cDNA libraries are constructed using messenger RNA (mRNA) expressed in different tissues.The expressed fragments of sequence data are not the full sequence of a known gene but what was incorporated into a mature mRNA molecule. EST sequences can be used for marker development in species in which the full genome has not been sequenced or assembled yet. Microsatellites developed from EST sequence have the advantage of being putative Type I markers (non-neutral), as it is possible to connect the function of the transcript of the genes from an EST sequence with the presence of a microsatellite.In contrast, 'traditional' microsatellites obtained from enriched libraries are generally Type II markers, which are not linked to genes of known function.