Time-resolved spectroscopic and microscopic measurements of membrane dynamics in living cells

摘要

Fluorescence spectra and lifetime images of cultivated CHO cells incubated with the membrane marker laurdan are reported. The plasma membrane and intracellular membranes were distinguished using illumination of either the cell surface (by an evanescent wave) or of the whole cell. Parameters of membrane dynamics, probed by the generalized polarization and the effective fluorescence lifetime of laurdan, were correlated with temperature, age and growth phase of the cells.