MICROMACHINED DMA MANIPULATION DEVICE USING CIRCULAR MULTI-ELECTRODE

摘要

Microfabricated DNA manipulation devices have a wide range of potential applications. In this paper, we present a new concept for efficient biological reaction and a DNA manipulation device with the reaction chamber, which consists of a center electrode and circular outer electrodes of a reaction unit. We verified the DNA manipulation concept by numerically simulating its operation, and experimenting with the fabricated device. This proposed complex micro reaction or micro analytical systems are generally composed of various components with different functionality within the micro DNA manipulation system. 2cm Square, entirely monolithic device has been fabricated with reaction chamber, circular type electrode, microfabricated heater and sensor. In the suggested reaction system, the charged bio-molecules, DNA, are manipulated by the charge of the electrode in a reaction unit. Controlling the induced dynamic electric field between the center electrode and the outer electrodes, Concentration (+2V) / Repulsion (-2V) / Manipulation (+1V～-1V) of bio-molecules are enabled at a periphery of electrode. It has no moving parts, physically constructed wall and can be built in a disposable unit of micro devices, which can be fabricated by conventional MEMS technique at low cost. Moreover, we evaluate temperature control unit characteristics for biological analysis such as PCR (Polymerase Chain Reaction), isothermal reaction temperature control. The coefficient of temperature heat resistance and heater temperature characteristic is about 0.0043 and 100°C /sec, respectively.