In Vitro Regeneration of Cardiovascular Tissues for Custom-made Tissue Transplantation

摘要

Decellularized tissues and those recellularization are widely studied to give more durability and invisible immunogenicity to the conventional bioprostheses. We are investigating efficient processes of decellularization and recellularization of biological tissues. Our recent studies on biomechanical properties of decellularized tissues were reported. Porcine pulmonary valves and aortas were excised and treated for 10 min by a newly developed method using ultrahigh pressure of 10,000 atm. They were then washed by PBS under microwave irradiation for 2 days. The vascular cells isolated from the future minipig recipient were injected into the aortic tissue by a dispenser and the endothelial cells were seed onto the surface of the decellularized tissue by a roller culture bioreactor. The cusps and aorta were completely cell free when new method was applied. There were no significant changes in biomechanical properties. The endothelial cells were seeded uniformly and the vascular cells were well injected to the decellularized scaffold. Biological tissues decellularized and recellularized in vitro by our new methods may provide more durable and safe bioprostheses.