Non-Invasive Structural Investigation of Renal Scaffold By Magnetic Resonance Imaging (MRI)

摘要

Naturally obtained scaffolds combined with patient-specific cells could result in improved regeneration of human tissues. Scaffold fabrication is the first step in this technology, which is decellularization of the native tissue and sterilization of resulting scaffold. Decellularization, as the most effective procedure, could be done by exposing the native tissue to cell lysing agents that remove all cellular materials leading to the intact collagenous structure of extracellular matrices (ECMs). The majority of cellular remnants should be withdrawn to avoid immune response induction while the ECM should remain intact. In complex organs such as the kidney, the ultrastructure is of significant importance since it is essential for the filtration function of the kidney. Examining the fabricated ECM for structural integrity and intactness of the vascular network was pursued in this study via a novel method.