On the Improvement of Immobilized Metal Affinity Chromatography (IMAC): Preparation, Characterization and Validation of a Superporous Agarose Monolith

摘要

Purification of biomolecules is a key step in the biotechnology industry as it often represents the major manufacturing costs. Affinity chromatography is a high performance purification step with high selective separation as it uses a reversible binding between biomolecules wanted to purify and a specific ligand joined to a chromatographic support. Immobilized metal ion affinity chromatography (IMAC) is a type of affinity chromatography based in the affinity of some side chain of amino acids for metal ions bound to a metal-chelating agent immobilized on a chromatographic matrix. Since the interaction between the immobilized metal ions and the side chain of amino acids has a reversible character, it can be utilized for adsorption and then be recovered using nondenaturing conditions.