The Effects of Viscosity on Nucleic Acid Assembly: A Possible Solution to the Strand Inhibition Problem

摘要

Increased control of molecular self-assembly is important for the further development of DNAbased technologies. While DNA technology research has been carried out almost exclusively in aqueous buffer, alternative solvents could increase our ability to direct DNA assembly. Deep eutectic solvents (DES), closely related to ionic liquids, are characterized by significantly lower melting points than their separate components. Recently, DES have been shown to preserve nucleic acid base pairing and duplex formation, offering a relatively unexplored medium for directing DNA assembly.