Assembly of G-quartet based DNA superstructures (G-wires)

摘要

G-wires are DNA superstructures based on the intermolecular interactions of four Guanine bases. They allow the fabrication of structures reaching the micrometer scale using only short DNA oligonucleotides, what makes them potentially interesting for molecular nanotechnology. We investigated the assembly of G-wires by SFM, using different sequences described in the literature. The assembled structures were adsorbed on mica and imaged by SFM. The influence of time and temperature of the growth was investigated, and the topology of the assemblies was studied.