Imaging of interferences between cellular particles and the fluorescence dye Merocyanine-540 during normoxia and anoxia

摘要

Subcellular particles (mitochondria, cellular pigments etc.) are mainly responsible for light scattering in living tissues in relation to the functional state. 2-D images clarify the respective tissue status during normoxia or anoixa such as the redox state of cytochromes. We realized tissue imaging of twelve isolated perfused rat livers stained with Merocyanine-540 during normoxia, hypoxia and anoxia. Merocyanine-spectra have shown a maximum fluorescence peak at 596 ± 2 nm. The optical response increased under desoxygenation and decreased under reoxyenation and might be correlated to electrical potential alterations. Futhermore, we record oscillations with frequencies over 7/sec (420/min) which might be correlated to intracellular processes. The additional use of dyes for tissue imaging gives us the opportunity of new insight into organ function.