Imaging of Green Fluorescent Protein in live plant by Scanning Near-field Optical Microscopy

摘要

An auxin/IAA induced in vivo green fluorescent protein (GFP) in a living plant Arabidopsis root has been studied by a scanning near-field microscope in transmission mode. The promising near-field images of the inducible GFPs at sub-surface of a plant cell suggest that they may locate proximity to the cell wall, i.e. both sides of and in the cytoplasm membrane. The clear and faint fluorescent spots with 1-3μm showed that the proteins localized nearer and farther to the cell wall, respectively. All GFP molecules gathered together in a cell, and no individual GFP was observed in the experiment.