The Ca~(2+)_i and MCLA-enhanced Ultraweak Chemiluminescence of Macrophages Irradiated with Low-intensity He-Ne Laser

摘要

Low-intensity He-Ne laser therapy (eg: intravascular laser irradiation on blood, ILIB) has been wildly used to treat with some illness in clinic, but the mechanism remains obscure. Laser power, the irradiation time and the irradiation dose were changed. Laser scanning confocal microscope (LSCM) was used to real-time research the effect of low-intensity laser irradiation on [Ca~(2+)]_i in mouse macrophages, and single photon counter device (SPC) was used to detect MCLA enhanced Chemiluminescence of macrophages irradiated with low-intensity He-Ne laser. Proper dose of low-intensity He-Ne laser irradiation can increase the [Ca~(2+)]_j of the macrophages and immunocompetence of macrophages effectively. Unsuitable dose of low-intensity He-Ne laser irradiation will not increase or even decrease the [Ca~(2+)]_i and immunocompetence of the macrophages. Laser power of 0.16mw is more effect to increase the [Ca~(2+)]_j than laser power of 0.40mw. The [Ca~(2+)]_i is markedly different even the irradiation dose or the irradiation time is the same. Both the irradiation dose and laser power are key factors for the change of [Ca~(2+)]_j and immunocompetence of macrophages. Activating immuno-system of body is one of the very important mechanisms of Low-intensity He-Ne laser therapy.