Preparation of magnetic composite microspheres by surfactant free controlled radical polymerization for cellulase immobilization

摘要

Magnetic composite microspheres consist of magnetic core and polymer shell have received much attention because of their wide range of potential applications in bioprocesses. Magnetic microspheres can be rapidly separated from the mixtures by a magnetic field and the polymer shell, which has some functional groups, can easily bind to many enzymes, protein, etc. Recently, magnetic composite microspheres has been increasingly used in immobilization of enzymes due to improved stability, the potential for modification of the catalytic properties, and in certain cases higher activity or selectivity. However, several methods have been reported for the preparation of magnetic composite microspheres. Most of microspheres cannot achieve high immobilization efficiency and the magnetite content is low, the distribution of magnetite in the polymer particles is not uniform.