Studies on Mechanisms of Insecticide Resistance in Blattella germanica

摘要

Blattella germanica has been a very good species for studying the mechanisms of resistance development. In this presentation we plan to describe our own efforts for elucidation of two cases of the resistance mechanisms in this species involving target insensitivities to two specific classes of insecticides: i.e. cyclodiene and kdr resistance. While the pattern of the shift of amino acids, resulting from mutation which specifically confer these resistance to B. germanica turned out to be identical to those simultaneously discovered in Musca domestica and Drosophila melanogaster, we found some differences in DNA base sequences and the nature of the sites specific mutations between B. germanica and these two dipteran species. One additional factor associated with the kdr phenomenon is the reduced mRNA expression of CaM-kinase. Since we could not find any mutation in the stretch of its DNA sequence which we could isolate, we conclude that this additional resistance factor is likely to be controlling the level of CaM-kinase expression.