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Alginate matrices to regulate granulosa cell morphology and steroid production

机译:藻酸盐基质调节粒细胞形态和类固醇生产

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The ability to mature granulosa cell-oocyte complexes (GOCs) in vitro may provide a mechanism to preserve the reproductive potential of women. Systems to mature GOCs in vitro must maintain the granulosa cell (OC)-oocyte interactions and coordinate the development of the oocyte. We propose that alginate based hydrogels can function as a synthetic stroma that maintains the three-dimensional architecture of the GOC. Additionally, the scaffold can regulate GC interaction with the extracellular matrix (ECM), which has been shown to affect differentiation, proliferation, and steroid production. Alginate was modified with minimal peptide sequences from ECM proteins. GCs attach and spread on peptide-modified surfaces, but not unmodified surfaces, and maintain proliferation rate. Progesterone production, an indicator of granulosa cell differentiation, increased up to a 3 fold on RGD modified alginate. These results indicate that the scaffolds can regulate GC differentiation, which will be a component of three-dimensional alginate scaffolds for in vitro maturation of GOCs. Additionally, this system provides an in vitro model to examine the role of cell-cell and cell-ECM interactions in tissue development.
机译:在体外成熟颗粒细胞 - 卵母细胞配合物(GOC)的能力可以提供一种保护女性的生殖潜力的机制。体外成熟的系统必须维持颗粒细胞(OC) - 卵细胞相互作用并协调卵母细胞的发育。我们提出基于藻酸盐的水凝胶可以作为保持GOC的三维架构的合成基质。另外,支架可以调节与细胞外基质(ECM)的GC相互作用,这已被证明会影响分化,增殖和类固醇产生。用来自ECM蛋白的最小肽序列修饰藻酸盐。 GCS在肽改性表面上附着和涂抹,但不制置的表面,并保持增殖率。黄体酮生产,颗粒细胞分化的指标,高达3倍的RGD改性藻酸盐。这些结果表明支架可以调节GC分化,这将是用于体外成熟的GOC的三维藻酸盐支架的组分。另外,该系统提供体外模型,以检查细胞 - 细胞和细胞-ECM相互作用在组织发育中的作用。

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