X-ray diffraction study on mouse stratum corneum

摘要

We investigated the structure of hairless mouse stratum corneum using X-ray diffraction with special attention to the difference between a control sample and a damaged sample. The treatment for getting a damaged sample was performed in such a way that mouse skin was exposed to sodium dodecyl sulfate (SDS) solution and the stratum corneum was separated from the mouse skin. By this procedure inflammation might take place. The control sample was the stratum corneum without the treatment. We found that X-ray diffraction pattern in the damaged sample is different from that in the control sample. Both in the small angle and wide angle X-ray diffraction patterns, in the damaged sample the peaks due to lipid lamellar and lipid packing are weaker than those in the control sample, respectively. Therefore, the deterioration of the lipid structure in the stratum corneum caused by the treatment can be distinguished by the X-ray diffraction.