Computed tomography of the periphery blood lymphocytes and erythrocytes

摘要

Many biolgoical objects, including blood cells, are otpically transparent. They don't absorb light and only change optical path length by variations of object's thickness or its refractive index variations. The phase shifting interferometry is used for the visualization of the phase information and the obtaining quantitative properties of cells. Cells are fixed by glutaraldehyde and mounted into immersion liquid for influences of refraction reduction. Blook cell's interferogram contains the iformation about space distriubtion refractive index. The phase-shift method was used for its reconstruction. This method includes record of four interferograms used different values of phase-shift of the reference wave. Many blood cells' interfereograms are obtained (lymphocytes and erythrocytes). The shape, size and density of cells are measurement. We suppose that these date will beones of the early diagnostically criteria of hematological diseases. Experimental results for interferometric microscopy and tomography of blood cells are presented.