Non-Invasive Studies of Proteins and DNA Fragments by SEC and SEC Coupling Methods

摘要

The combination of GPC/GFC/SEC separation with light scattering detection is a promising method to study the interactions of proteins and protein-ligand complexes. GPC/SEC-MALLS is non- invasive and non-destructive. The samples need not be derivatized by radioactive or fluorescent labels. It is possible to recover proteins after the experiment for subsequent investigation. Comprehensive results are obtained when selecting highly efficient columns for sample fractionation in combination with comprehensive detection techniques like multi-angle laser light scattering detection (MALLS). This paper describes strategies for column selection and method hyphenation for various proteins and DNA fragments and shows results which would be much more cumbersome and time consuming to obtain otherwise.