Differences in PDMS Modification Affect Laminin Deposition and Smooth Muscle Cell Response

摘要

When culturing cells on flexible surfaces, it is important to consider extracellular matrix treatments that will remain on the surface under mechanical strain. Here we investigate differences in laminin surfaces that were deposited on oxidized polydimethylsiloxane (PDMS) (plasma only) versus oxidized PDMS treated with aminopropyltrimethoxysilane (silane-linked). We use specular X-ray reflectivity (SXR), transmission electron microscopy (TEM), and immunofluorescence to probe the quantity and uniformity of the laminin on the treated PDMS surfaces. We also compare vascular smooth muscle cell (SMC) proliferation on laminin under equibiaxial strain between plasma only and silane-linked treatments.