The interdependence of C and N metabolism in achieving maximum reproductive development of maize is well known, but poorly understood. Our experimental approach has been to use stem infusion and in vitro kernel culture to control and/or abruptly change the supply of C and N assimilates available for ear and kernel development. A major goal of this research has been to ascertain whether kernel growth and composition is determined by the assimilate supply from the vegetation, or by factors operating within the kernel. For in vitro grown kernels, endosperm sugar status was closely related to C supply, and a supply on N allowed the kernel to use this assimilate for growth.
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