Optical diamagnetic biosensor for immunocomplexes on beads

摘要

In the present work diamagnetic separation parameters for the porous beads are studied using optical videorecording microscopy. The possible direct amount determination of single or double macromolecular layers immobilised in the meshes of the porous beads is demonstrated for the concentrations' range used in heterogenic immunotests and the affinity chromatography, where the direct rapid detection of ligands within sorbent particles is known to be the actual task. A gradient diamagnetic biosensor is described as suitable for rapid quantitative detection of single or double macromolecular layers in porous nonmagnetic beads. Measurements of capture travelling time or accumulation radius in gradient magnetic field have shown that it is possible to determine 0.20 mg/ml of macromolecular amount within several seconds. The portative devices were made on the base of the fabre optic technique to detect accumulation radius of collected beads in two gradient magnetic positions: diamagnetic and paramagnetic zones of magnetised wire with 55 μ m in diameter and to registrate with a lot of fabre wires having 30 μ m in diameters. The successive procedures of the present method can be described by: the obtaining of agarose immuno-beads, the incubation of beads with the ligand sample or the injection of sample through affinity mini- column, the submerging of the loaded beads into the glass cell containing Ni-wire or the narrow gap of magnetic poles; the computational obtaining of immuno-parameters: binding constants, accumulation radius. Several biotechnological applications of the biosensor are presented on sorbent beads, human lymphocytes.