Dynamic binding performance of large biomolecules such as γ-globulin, viruses and virus-like particles on various chromatographic supports

摘要

Dynamic binding (adsorption) performance of γ-globulin, several viruses and virus-like particles was examined with various types of ion-exchange chromatography (IEC) supports. Among various IEC supports, a sulfated cellulose bead adsorbent (Cellufine-Sulfate) was carefully investigated as this is known to show unique bioaffinity and size exclusion behavior. Size exclusion curves and HETP-flow rate curves under non-binding conditions showed that proteins and viruses are totally excluded from Cellufine-Sulfate beads, and they can adsorbe only on the surface layer of the bead. Biospecificity to Cellufine-Sultate was tested with various proteins, viruses and virus-like particles. Dynamic adsorption capacities (DBC) were determined as a function of flow-rate. DBC of γ-globulin on Cellufine-Sulfate did not depend on the flow velocity whereas conventional porous agarose-based IEC supports show a very strong flow-velocity dependent DBC.