Synthesis and Application of Titanate Nanotubes in Lactate Electrochemical Biosensor

摘要

Hydrogen titanate (H{sub}2Ti{sub}3O{sub}7) nanotubes (TNTs) have been synthesized by a one-step hydrothermal reaction of TiO{sub}2 with NaOH. A reagentless amperometric lactate oxidase (LO{sub}x)-based biosensor has been constructed for lactate detection. In the biosensor, the LO{sub}x enzyme is immobilized on to the TNTs that are multiwall hollow tubes with two open ends with diameter of 15-20 nm. The biosensor exhibits a sensitivity of 4 μA/mM and a linear response range up to 14 mM lactic acid. The TNTs facilitate direct electron transfer between the active centers of LO{sub}x and the gold electrode.