PROTOCOL CHANGES FROM THE STANDARD M. PARATUBERCULOSIS CULTURE METHODS TO INCREASE THE SENSITIVITY OF JOHNE'S DIAGNOSIS IN BEEF CATTLE

摘要

Johne's Disease is caused by Mycobacterium avium subspecies paratu-berculosis (MAP). This disease affects cattle and other ruminants. After animals become infected, this bacterium will eventually target the mesen-teric lymph nodes and intestines of animals. Animals infected with MAP develop chronic wasting, diarrhea with shedding of these bacteria in the feces, and, eventually death. More is known about Johne's infection in dairy cattle than beef cattle. The majority of serology and culture assays used to identify MAP positive cattle are from dairy herd studies.