Multifrequency phase-resolved fluorescence spectroscopy is used to generate a multidimensional data array in which phase-resolved fluorescence intensity is measured as a function of emission wavelength and excitation wavelength at a series of excitation modulation frequencies. The dependence of the phase-resolved fluorescence intensity on fluorescence lifetime, modulation frequency and detector phase angle setting is examined. A model two-component system of benzo(k)fluoranthene and benzo(b)fluoranthene has been used to demonstrate the ability of phase-resolved fluorescence spectroscopy to selectively enhance the spectral contribution of a component as a function of fluorescence lifetime in total luminescence spectroscopy, and is also discussed here.
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