Free Calicium Distribution and Calcium Pulses in Rat Peripheral Macrophages

摘要

With Laser Confocal Scanning Microscope (LCSM) system, three aspects of characteristics of free cytoplasmic calcium in rat peripheral macrophages are studied. One is the Ca~2+ concentration in different area in the same cell. Second is the Ca~2+ concentration in the same area in different dividing stage. Third is the feature of calcium pulses evoked by KCl or pH changing. The results show that even in one cell, the evolution of the Ca~2+ concentration is not same in different area. I nthe same area, the nucleolus Ca~2+ concentration in division breaking stage is much higher than that in division stage. From the experiment phenomena, we conclude that KCl itself can not evoke calcium pulses in the unexcitable macrophage, but the change of pH can trig calcium pulses in the same cells.