Occurrence of Rhizobium phaseoli phages in minia soils

摘要

Seven soil samples were collected from the rhizosphere of Phaseolus vulgaris grown in Minia soils. Presence of rhizobiophages specific for Rhizobium phaseoli as well as properties of such phages, 1. e. plaque morphology, host range, size and morphology of the particles were studied in the collected samples. Four Rhizabium phaseoli strains (two local isolates L1, L2 and two American strains 2669 & 2674) were used for soil enrichment and as indicator bacteria. The obtained results indicated that phagesof R. phaseoli were detected in all the collected samples. Fifty three phage isolates showing different plaque morphology were isolated randomly from the seven soil samples. Diameters of the formed plaques ranged from 1 to 4 nun and their appearance varied from turbid to clear.