Risk Assessment of Endocrine Disrupting Chemicals in Aquatic Environment Using Cultured Mammalian Cells

摘要

For development of monitoring models, the effects of 4-nonylphenol, which has been reported to act in an identical way to 17β-estradiol in stimulating receptor transcription, were studied using cultured mammalian cells. The enzyme alteration, DNA changes, and gene expression as biomarkers on human breast cancer cell line MCF-7 and human intestinal cell line Caco-2, which derived from estrogen targeted or non-targeted tissues were examined. MCF-7 cells treated with 4-nonylphenol at the concentration of 10 μM increased significantly. During 24 h, treatment with 4-nonylphenol at the concentration of 10 μM increased the expression of estrogen receptor protein in MCF-7 cells. The transepithelial electrical resistance (TEER) value of the Caco-2 cell monolayer was significantly decreased by incubation for 60 min with 4-nonylphenol at the concentration of 10~(-8) M, and induced DNA fragmentation of Caco-2 cells.