- Liposome-aptamer complex for removing endocrine disrupting chemicals and method for removing endocrine disrupting chemicals by using the same

摘要

A liposome-aptamer complex is provided to remove endocrine disrupting chemicals effectively by positioning the hexane aptamer in a submicron-sized liposome consisted of a bilayer lipid membrane so that a hexane aptamer peculiarly bonded to endocrine disrupting chemicals can be used in an actual underwater environment, thereby bonding well the hexane aptamer to the endocrine disrupting chemicals, and a method for removing endocrine disrupting chemicals in an underwater environment by using the liposome-aptamer complex is provided. A liposome-aptamer complex for removing endocrine disrupting chemicals is characterized in that a hexane aptamer peculiarly bondable with endocrine disrupting chemicals is introduced into a liposome consisted of a bilayer lipid membrane. A preparation method of a liposome-aptamer complex for removing endocrine disrupting chemicals comprises the steps of: developing an aptamer perculiar to target endocrine disrupting chemicals by an SELEX(Systematic Evolution of Ligands by Exponential enrichment) method; dissolving a single lipid material into chloroform, evaporating the dissolved solution to form a dilayer lipid membrane, injecting a buffer capable of being bonded optimally to the endocrine disrupting chemicals into the dilayer lipid membrane, and forming a liposome from the mixture using a sonicator; mixing the aptamer and the liposome in a tris bubber; mixing an ethanol/calcium chloride solution with the mixed solution, and dialyzing the mixed solution with a PBS solution; and purifying a liposome-aptamer comple using a high speed centrifuge.