Synthesis of amino acids selectively labelled with stable isotopes for application in peptide synthesis

摘要

Amino acids labelled with stable isotopes such as ~2H, ~13C and ~15N were originally applied in metabolic studies and have subsequently played an important role also in many biosynthetic and mechanistic investigations. With the growing use of ~13C and ~15N NMR spectroscopy in strutural studies of proteins, isotope labelling has often become a major tool to increase the sensitivity as the natural abundance of these nuclei is only 1.1 and 0.35