DEVELOPMENTS IN BAGASSE SPORE DETECTION METHODS

摘要

An immunofluorescent microscopic method for specifically detecting and counting airborne Thermoactinomyces sacchari spores has been developed. The spores were indirectly labelled with (FITC) fluorescein isothiocyanate. The method has been successfully field tested during stored bagasse reclamation, when the downwind drift of airborne spores from the truck loading point was monitored. High airborne spore levels were detected close to the loading point, although it was apparent that local topography and weather conditions, particularly wind strength and direction, had significant influence over the extent and direction of drift. In addition, an ELISA blood test has been developed as an aid to diagnosis of suspected bagassosis. Medical practitioners in cane growing areas will have access to the ELISA, along with information on other diagnostic criteria and case management. The development of the ELISA, used in conjunction with other tests such as lung function, will enable large scale surveillance to be undertaken.