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Improved no-moving-parts video-rate confocal microscope

机译:改进的无运动零件视频速率共聚焦显微镜

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Several years ago our research program developed a video-rate confocal microscope with no moving parts, based on synchronizing and aligning the scan of an image dissector tube (IDT) with the return light resulting from an acousto-optically scanned laser beam. Improvements on the original system have recently been completed. The laser scan is now brought into the Nikon Diaphot inverted microscope through the epi-illumination port and the laser power has been substantially increased. All beam shaping in the laser scanner is done with prisms instead of cylindrical lenses to reduce aberrations. The IDT is located at the side video output port at the end of a very efficient light path. The new system is described along with some results obtained in the laboratory.
机译:几年前,我们的研究计划开发了一种视频速率共聚焦显微镜,没有基于同步和对准由声光扫描激光束产生的返回光的图像分析管(IDT)的扫描来对准。最近完成了原始系统的改进。现在,激光扫描通过ePI照明端口进入尼康二射反转显微镜,并且激光功率显着增加。激光扫描仪中的所有光束都是用棱镜完成而不是圆柱形透镜来减少像差。 IDT位于非常有效的光路的末端的侧视输出端口。新系统将描述在实验室中获得的一些结果。

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