Systolic and diastolic global right and left ventricular function assessment in small animals using an automated angiographic technique

摘要

To assess left ventricular (LV) and right ventricular (RV) systolic and diastolic function in rats and mice, the authors developed a field-by-field angiographic analysis program. Interlaced video images were acquired on a videotape recorder under a constant fluoroscopic technique, and digitized at 512*512, 8-b, 30-frames/s resolution. Fields were separated to improve the temporal resolution. Field-by-field video-density curves for LV and RV were derived. Beat averaging was performed after correcting for varying contrast between beats. The following parameters were measured: (1) heart rate, (2) ejection fraction, (3) end diastolic volumes, (4) stroke volume, (5) cardiac output, (6) mean systolic ejection rate, (7) peak ejection rate, (8) peak filling rate, (9) time to peak filling, and (10) time to peak ejection rate.