Self-interference digital holography (SIDH) is a promising approach for three-dimensional imaging as it o ersthe ability to view a complete three-dimensional volume from a single image. SIDH has so far, largely beenlimited to image samples that emit a large number of photons. We report the use of a Michelson interferometerbased SIDH setup which provides higher light throughput compared to previous systems that employed spatiallight modulators (SLM). SIDH microscopy incorporating a Michelson interferometer in the proposed opticalcon guration and high-numerical-aperture oil immersion objectives can be used to perform super-resolutionsingle-molecule localization microscopy (SMLM) and single-particle tracking (SPT) over large axial ranges. Wedemonstrate this by localizing a single 0.1 m diameter uorescent nanosphere using a custom built wide- eldmicroscope. With 49,000 photons detected, the proposed system achieves a localization precision of 4.5 nm in x,5 nm in y and 39.8 nm in z over a 20 m axial range. Further, we also discuss the SNR requirements to imagephoton-limited light sources such as a single-molecules using SIDH.
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